Orthogonal Array Design for the Optimization of Pepsin Soluble Collagen Extraction from Cyprinus carpio haematopterus Scale

doi:10.7506/spkx1002-6630-201512011

Abstract

Abstract:

Pepsin and 0.5 mol/L acetic acid were used to enhance the extraction of collagen from Cyprinus carpio
haematopterus scale at 4 ℃. Using combination of single factor experiments and orthogonal array design, the effect of
extraction time, enzyme concentration and ratio of solid to solvent on the yield of pepsin soluble collagen (PSC) from
C. haematopterus scale were explored. The results showed that the optimal conditions for PSC extraction were determined
as follows: extraction time, 60 h; enzyme concentration, 40 kU/g; and ratio of solid to solvent, 1:10 (g/mL). The yield of
PSC under these conditions was (17.7 ± 0.7)%. The PSC had electrophoretic properties amino acid composition similar to
those of acid soluble collagen (ASC), suggesting similarity beween their molecular structures.

Key words: Cyprinus carpio haematopterus scale, collagen, extraction process

CLC Number:

TS254.9

XIAO Feng1,2, ZHU Wenxue1,*, KANG Huaibin1, LIU Lili1, HE Jialiang1, REN Guoyan1. Orthogonal Array Design for the Optimization of Pepsin Soluble Collagen Extraction from Cyprinus carpio haematopterus Scale[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201512011.

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Orthogonal Array Design for the Optimization of Pepsin Soluble Collagen Extraction from Cyprinus carpio haematopterus Scale

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