Separation and Purification of Citrus Limonin by Macroporous Resin

doi:10.7506/spkx1002-6630-201514005

Abstract

Abstract:

A method for the purification of limonin from citrus by macroporous resin was proposed. The results showed that
D101 resin was the best adsorbent for the purification of citrus limonin. The optimal purification conditions were achieved
by loading the sample containing 0.5 mg/mL limonin onto the column at a flow rate of 1 mL/min and subsequently eluting
the adsorbed limonin with 80% alcohol at pH 6 at a flow rate of 2 mL/min. The adsorption capacity of D101 resin remained
almost unchanged after several cycles of repeated use and regeneration. Under the optimized conditions, the adsorption rate,
desorption rate and yield of limonin were 88.53%, 93.47% and 82.75%, respectively. The result of HPLC showed that the
limonin content (i.e., purity) of the purified product was 92.79%.

Key words: citrus, limonin, D101 macroporous resin, separation, purification

CLC Number:

TS201.1

PANG Li, DENG Ziniu, LIU Jikai, WANG Rencai*. Separation and Purification of Citrus Limonin by Macroporous Resin[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201514005.

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Separation and Purification of Citrus Limonin by Macroporous Resin

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