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In vitro Anti-inflammatory Effect of Total Flavonoids from Blueberry Leaves

ZHAO Dan1, SU Ning2, YANG Li2, ZHENG Hongyan2, HUO Tong1, WANG Changtao1,*   

  1. 1. School of Science, Beijing Technology and Business University, Beijing 100048, China;
    2. Chinese Academy of Inspection and Quarantine, Beijing 100176, China
  • Online:2015-09-15 Published:2015-09-11


Purpose: To evaluate the in vitro anti-inflammatory potential of total flavonoids from blueberry leaves. Methods:
a co-culture system of HaCaT cell and flavonoids were constructed. To examine the effect of flavonoids on the expression of
inflammatory cytokines including interleukin 6 (IL-6), interleukin-8 (IL-8), and interferon induced protein 10 (IP-10), realtime
reverse transcription quantitative PCR (RT-qPCR) was used to detect the expression of the related genes after co-culture
for different periods of time(0, 15, 30, 60, and 120 min). The anti-inflammatory mechanism of flavonoids from blueberry
leaves was analyzed at the molecular level. Results: The total flavonoids from blueberry leaves significantly enhanced
the expression of anti-inflammatory cytokines (IL-6 and CXCR-2) (P < 0.05) and significantly inhibited the expression of
pro-inflammatory cytokines (IL-8, IP-10) (P < 0.05) to achieve the anti-inflammatory activity. Chemotactic factor receptor
CXCR-1 was regulated complicatedly. Conclusions: The total flavonoids from blueberry leaves could decrease inflammation
by regulating the function of several gene loci via several steps.

Key words: total flavonoids from blueberry leaves, inflammatory cytokines, real-time reverse transcription PCR, anti-inflammatory

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