Determination of Proanthocyanidins in Lotus Seed Shell Extract by HPLC

doi:10.7506/spkx1002-6630-201502028

Abstract

Abstract:

Objective: To establish a high performance liquid chromatography (HPLC) method for the determination of
proanthocyanidins in lotus seed shell extract. Methods: HPLC analysis was carried out using Diamonsil C18 (4.6 mm × 250 mm,
5 μm), and the mobile phase consisted of methanol-water-formic acid-isopropyl alcohol (13:73:8:6, V/V) at a flow rate of
1.0 mL/min. The detection wavelength was set at 525 nm, the column temperature was set at 35 ℃, and the injection volume
was 10 μL. Results: A good linearity of proanthocyanidins was achieved in the range of 37.6–300.8 μg/mL (r = 0.999 5).
The average recovery was 99.2%, with relative standard deviation (RSD) of 0.30%. Conclusion: This method is simple,
reproducible, accurate and reliable and thus can be used for quality control of lotus see shell extract.

Key words: lotus seed shell extract, proanthocyandins, high performance liquid chromatography (HPLC)

CLC Number:

O657.7

TONG Xidi, PENG Hong*, WANG Qiong, JIN Xin. Determination of Proanthocyanidins in Lotus Seed Shell Extract by HPLC[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201502028.

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Determination of Proanthocyanidins in Lotus Seed Shell Extract by HPLC

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