Extraction, Purification and Qualitative Analysis of Proanthocyanidins from Pitaya Peel

doi:10.7506/spkx1002-6630-201502014

Abstract

Abstract:

The crude proanthocyanidins extracted from pitaya peel were purified by macroporous adsorption resin. AB-8
macroporous resin was selected as the best adsorbent for proanthocyanidins based on adsorption and desorption capacities in
comparison with DM130 and ADS-17. Effects of sample loading flow rate, elution flow rate and eluent concentration on the
purification of pitaya peel proanthocyanidins by AB-8 macroporous resin were investigated. The best results were achieved
by sample loading at a flow rate of 2 mL/min followed by elution with 50% ethanol at a flow rate of 1 mL/min. The purified
product contained 96.65% proanthocyanidins including catechin, epicatechin and dimmer as indicated by colorimetric
observation and qualitative analysis by high performance liquid chromatography-electrospray ionization-mass spectrometry
(HPLC-ESI-MS).

Key words: pitaya peel, proanthocyanidins, extraction and purification, qualitative analysis

CLC Number:

TS201.2

YANG Zhijuan, ZENG Zhen*, WU Xiaoping. Extraction, Purification and Qualitative Analysis of Proanthocyanidins from Pitaya Peel[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201502014.

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Extraction, Purification and Qualitative Analysis of Proanthocyanidins from Pitaya Peel

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