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Separation and Purification of Polysaccharides from Ma Bamboo Shoots (Dendrocalamus latiflorus)

WU Jinsong1,2, ZHENG Jiong1, XIA Xuejuan1, WANG Jian3, KAN Jianquan1,*   

  1. 1. College of Food Science, Southwest University, Chongqing 400715, China; 2. College of Food Science and Engineering,
    ZhengZhou University of Science and Technology, Zhengzhou 450064, China;
    3. Chongqing Institute for Food and Drug Control, Chongqing 401121, China
  • Online:2015-01-25 Published:2015-01-16
  • Contact: KAN Jianquan

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Abstract:

The aim of this study was to optimize the separation and purification of polysaccharides from Ma bamboo shoots
(Dendrocalamus latiflorus). Crude polysaccharides were extracted from bamboo shoots by hot water extraction method,
deproteinized, decolorized by dialysis and fractionated by DEAE-52 cellulose and Sephadex-50 column chromatography.
The results showed that papain treatment combined with Sevag method was the best deproteinization condition. Three major
polysaccharide fractions were eluted from DEAE-52 cellulose with water, 0.05 mol/L NaCl and 0.1 mol/L NaCl, namely
BSP1, BSP2 and BSP3, respectively. The fractions were further purified by Sephadex-50 column chromatography to produce
BSP1A, BSP2A and BSP3B, respectively. These further purified polysaccharides were found to contain almost no protein or
nucleic acid with a purity more than 95.3%.

Key words: bamboo shoot polysaccharide, deproteinization, dialysis, fractionation, purity identification

CLC Number: