Production of Functional 1,3-Diacylglyerol from Monoacylglycerol by Lipase-Catalyzed Transesterification

doi:10.7506/spkx1002-6630-201522001

Abstract

Abstract:

sn-1,3-Diacylglycerol (sn-1,3-DAG) was synthesized from glycerol monooleate (GMO) by the immobilized
Thermomyces lanuginosus lipase (Lipozyme TLIM)-catalyzed esterification in a solvent-free system. The effects of different
types of acyl donors such as conjugated linoleic acid (CLA-FA) and conjugated linoleic acid ethyl ester (CLA-EE), reaction
time and molar ratio of substrates on acyl migration and glyceride concentration were investigated to improve the yield of
sn-1,3-DAG. of the yield of sn-1,3-DAG was 65% when the reaction proceeded at a TLIM concentration of 20% by mass
with a molar ratio of conjugated linoleic acid ethyl ester (CLAEE):GMO of 3:1 for 2 h at 50 ℃ in a water bath shaker at
220 r/min. Also, the effects of two types of acyl donors (free acid and ethyl ester) on acyl migration and the yield of sn-1,3-
DAG were compared. This study can provide a reference for the industrial production of sn-1,3-DAG.

Key words: transesterification, conjugated linoleic acid, conjugated linoleic acid ethyl ester, glyceryl monooleate, lipase TLIM

CLC Number:

TQ225.24

HUANG Chuchu, XIONG Huihuang, GONG Bin, YU Yun, FENG Yue, LIANG Yuan, ZHU Xuemei. Production of Functional 1,3-Diacylglyerol from Monoacylglycerol by Lipase-Catalyzed Transesterification[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201522001.

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Production of Functional 1,3-Diacylglyerol from Monoacylglycerol by Lipase-Catalyzed Transesterification

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