Abstract:

The inclusiveness, exclusivity, sensitivity, specificity, accuracy, limit of detection (LOD), resistance
to denaturation and batch variation of the commercial Salmonella nucleic acid detection kit used to detect Salmonella in food
samples were evaluated based on indoor technical parameters by validation and collaboration test. Results showed that the
kit was not significantly different from the national standard method in general, and could meet the requirements of food
sample testing. However, the sensitivity for vegetables products was lower than those of other foods, which significantly
differed from the national standard method. Thus the commercial kit was not suitable for detection of Salmonella in
vegetable products.

Key words: Salmonella, nucleic acid detection kit, loop-mediated isothermal amplification, evaluation