FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (11): 1-7.doi: 10.7506/spkx1002-6630-201811001

• Basic Research •     Next Articles

Determination of Thermal Hysteresis Activity of Antifreeze Protein from Antarctic Krill (Euphausia superba) by Differential Scanning Calorimetry

MA Qingbao1,2, LIU Zhidong1,*   

  1. 1. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; 2. College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China
  • Online:2018-06-15 Published:2018-06-06

Abstract: In this study, we investigated whether differential scanning calorimetry (DSC) can be used to detect thermal hysteresis activity (THA) of antifreeze proteins (AFPs) from Antarctic krill. Important experimental conditions, such as cooling rate, sample concentration, ice crystal content and buffer system, were optimized. The results showed that the molecular weight of AFPs was about 76 kDa and its THA was 1.76 ℃. A cooling rate of 1.00 ℃/min, a sample concentration of 1.0 mg/mL and an ice crystal content ranging from 10% to 15% were found to be the optimal conditions. THA was greatly affected by different buffer systems. This study led us to conclude that DSC can be effectively used for the evaluation of THA of AFPs.

Key words: Euphausia superba, antifreeze protein, thermal hysteresis activity, differential scanning calorimetry, evaluation

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