FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (2): 260-266.doi: 10.7506/spkx1002-6630-201802041

• Processing Technology • Previous Articles     Next Articles

Optimization of Microwave-Assisted Aqueous Enzymatic Extraction of Cyclic Adenosine 3’,5’-Monophosphate (cAMP) from Chinese Jujube (Ziziphus jujuba Mill.) and Its Anti-Fatigue Effect

REN Yanrong1,2, ZHANG Zhongming3, XIE Yuejie1, XIONG Zhengwei1,2, WANG Zhongming1, WU Hongbin4,*   

  1. (1. College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China; 2. Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China; 3. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 737100, China; 4. Institute of Agro-Food Science and Technology, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China)
  • Online:2018-01-25 Published:2018-01-05

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Abstract: Response surface methodology was used to optimize the microwave-assisted aqueous enzymatic extraction of cyclic adenosine 3’,5’-monophosphate (cAMP) from Chinese jujube (Zizyphus jujuba Mill.) grown in Xinjiang. The anti-fatigue effect of the cAMP extracted was investigated. The optimal extraction conditions was found as follows: enzyme concentration 1.5% (a mixture of cellulase, hemicellulase and pectinase, 1:1:1 (m/m)), temperature 31.3 ℃, time 20 min and microwave power 500 W. Under these conditions, the yield of cAMP was 560.9 μg/g. The results showed that the cAMP could extend exhaustive swimming time and enhance swimming endurance in mice, suggesting good anti-fatigue effect. Therefore, cAMP holds great promise for its application in the food and pharmaceutical industry.

Key words: Zizyphus jujuba, cyclic adenodsine 3’,5’-monophosphat (cAMP), microwave-assisted aqueous enzymatic extraction, swimming time

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