Abstract: The conditions for extracting volatile components from Flos Lonicerae by headspace solid-phase microextraction (HS-SPME) were optimized by one-factor-at-a-time and orthogonal array design methods. Gas chromatography-mass spectrometry (GC-MS) and gas chromatography-quadrupole time-of-flight mass spectrometry (GC-QTOF MS) were used to detect the extracts obtained by HS-SPME and steam distillation (SD). The results showed that the optimum conditions of HS-SPME were as follows: extraction temperature 60 ℃, equilibrium time 25 min, extraction time 60 min, and desorption time 5 min. Under these conditions, 59 components were identified, accounting for 97.17% of the total amount, and the main components extracted by HS-SPME were terpenes, alcohols and aldehydes; a total of 68 components were identified from the SD extract, accounting for 91.69% of the total amount, with alcohols, aldehydes and ketones being dominant. Accordingly, the types and contents of volatile oil components extracted by the two methods were quite different. This study provides a scientific basis for quality identification, development and utilization of Flos Lonicerae.

Key words: Flos Lonicerae, headspace solid-phase microextraction, steam distillation, volatile components