FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (4): 178-184.doi: 10.7506/spkx1002-6630-20181029-343

• Component Analysis • Previous Articles     Next Articles

Comparison of Volatile Components from Flos Lonicerae Extracted by Headspace Solid Phase Microextraction and Steam Distillation

ZHANG Pengyun, GUAN Wei, LI Rong, LONG Chunxia, LU Junwen, LIN Shumian, ZHANG Feng   

  1. (1. Technology Center, Zhongshan Customs, Zhongshan 528400, China; 2. Luoyang Center for Disease Control and Prevention, Luoyang 471000, China; 3. School of Public Health, Guangdong Pharmaceutical University, Zhongshan 528458, China; 4. Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100176, China)
  • Online:2020-02-25 Published:2020-03-02

Abstract: The conditions for extracting volatile components from Flos Lonicerae by headspace solid-phase microextraction (HS-SPME) were optimized by one-factor-at-a-time and orthogonal array design methods. Gas chromatography-mass spectrometry (GC-MS) and gas chromatography-quadrupole time-of-flight mass spectrometry (GC-QTOF MS) were used to detect the extracts obtained by HS-SPME and steam distillation (SD). The results showed that the optimum conditions of HS-SPME were as follows: extraction temperature 60 ℃, equilibrium time 25 min, extraction time 60 min, and desorption time 5 min. Under these conditions, 59 components were identified, accounting for 97.17% of the total amount, and the main components extracted by HS-SPME were terpenes, alcohols and aldehydes; a total of 68 components were identified from the SD extract, accounting for 91.69% of the total amount, with alcohols, aldehydes and ketones being dominant. Accordingly, the types and contents of volatile oil components extracted by the two methods were quite different. This study provides a scientific basis for quality identification, development and utilization of Flos Lonicerae.

Key words: Flos Lonicerae, headspace solid-phase microextraction, steam distillation, volatile components

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