Abstract: In this study, chitosan, vanillin and polyvinyl alcohol were used as raw materials to prepare chitosan/vanillin/polyvinyl alcohol (CS/V/PVA) composite electrospun nanofiber film by uniaxial electrospinning. The morphology and structure of CS/V/PVA were characterized by field emission scanning electron microscopy (SEM), Fourier infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorimeter (DSC) and thermogravimetric analyzer (TG). The antibacterial activity was determined against Shewanella putrefaciens, the dominant spoilage bacterium in turbot. In addition, the application of CS/V/PVA composite electrospun nanofiber film was evaluated to the preservation of turbot. The results showed that CS/V/PVA composite electrospun nanofiber film had a good microstructure, and the fiber diameter was evenly distributed between 200 and 350 nm. A strong interaction between the components of the nanofiber film was observed. The aldehyde group in the vanillin molecule could react with the amino group in chitosan to form Schiff base bonds, and hydrogen bonds were formed between the chitosan, vanillin and polyvinyl alcohol molecules, reducing the initial melting temperature of the film. In addition, the co-spinning membrane had a strong antibacterial ability against Shewanella putrefaciens, and at 4 ℃ it could prolong the shelf life of turbot at 4 ℃.

Key words: electrospinning; turbot; chitosan; vanillin; Shewanella putrefaciens