Abstract:

High-performance liquid chromatography with fluorescence detection (HPLC-FLD) and high performance liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry (HPLC-APCI-MS/MS) were used for analyzing amitorle residue in plant-derived foodstuffs. In HPLC-FLD analysis, the excitation wavelength and emission wavelength were set at 380 and 484 nm, respectively. HPLC-APCI-MS/MS analysis was carried out in the MRM mode under the conditions as follows: parent ion, m/z 85; quantitative daughter ion, m/z 43; qualitative daughter ion, m/z 57; collision energy, m/z 85 > m/z 57 to 56.9/11 eV, m/z 85 > m/z 43 to 58.1/11 eV; and cracking voltage, 100 V. Limit of detection was 0.01 mg/kg for HPLCFLD method and 0.005 mg/kg for HPLC-APCI-MS/MS method. Both methods demonstrate the advantages of simplicity, rapidity, accuracy and reliability and are alternatives to each other for the determination the determination of amitorle residue in plant-derived foodstuffs.

Key words: high performance liquid chromatography with fluorescence detection (HPLC-FLD), high performance liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry (HPLC-APCI-MS/MS), amitorle residue