Abstract:

With the aim of achieving an in-depth understanding of effect of ultra-high hydrostatic pressure treatment on limonene in navel orange juice, a mathematical model describing the relationship between limonene content and three treatment parameters, such as pressure(100－500 MPa), temperature (20－40 ℃) and treatment time (10－20 min) was created using Box-Behnken central composite design combined with regression analysis, and interactive effects of the three variables on the dependent variable were then evaluated using response surface methodology (RSM). This model was found to be significant (P＜ 0.0001) with a regression coefficient of 0.9938 and an adjusted regression coefficient of 0.9858. The predicted values of limonene content were in good agreement with the experimental values. This demonstrated the reliability of the created model in predicting limonene content in navel orange juice subjected to ultra-high hydrostatic pressure treatment under different conditions.

Key words: ultra-high hydrostatic pressure, orange juice, limonene, RSM