FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (1): 240-242.doi: 10.7506/spkx1002-6630-201001056
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DING Cheng-shi
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Abstract:
Objective: To isolate and purify glycoprotein from Gastrodia elata (GGE2b) and investigate its hemorrheological effect in acute blood stasis rats. Methods: Soluble extracts of Gastrodia elata were purified by DEAE-Sepharose column chromatography and fast protein liquid chromatography (FPLC) to obtain GGE2b. Acute blood stasis rat model was developed by hypodermic adrenaline injection combined with cold-shock treatment. Totally 60 male rats were randomly divided into 6 groups of 10 rats each, namely saline group, acute blood stasis model group, PGE2b administration groups at doses of 30, 60 and 120 mg/kg, and positive control group administered with Danshen injection. Effect of GGE2b on whole-blood viscosity, plasma viscosity, red blood cell (RBC) accumulation, RBC aggregation index and RBC deformability index in acute blood stasis rats was examined. Results: GGE2b was successfully purified from Gastrodia elata through combined technology of DEAE-Sepharose column chromatography and FPLC. Moreover, GGE2b administration at the doses of 60 mg/kg and 120 mg/kg in rats resulted in a dramatic decrease of plasma viscosity (P < 0.05), whole-blood viscosity (P <0.01) and RBC aggregation index (P< 0.05), and increase of RBC deformability index (P <0.05). Conclusion: It is feasible to purify glycoproteins from Gastrodia elata using chromatography technology. GGE2b can result in a significant improvement of abnormal hemorrheology in rats with acute blood stasis.
Key words: glycoprotein from Gastrodia elata, fast protein liquid chromatography, acute blood stasis, hemorrheology
CLC Number:
TQ929.2
DING Cheng-shi. Isolation, Purification and Hemorrheological Effect of Glycoprotein from Gastrodia elata (GGE2b) in Blood Stasis Rats[J]. FOOD SCIENCE, 2010, 31(1): 240-242.
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URL: https://www.spkx.net.cn/EN/10.7506/spkx1002-6630-201001056
https://www.spkx.net.cn/EN/Y2010/V31/I1/240