Isolation, Purification and Anti-tumor Activity of a Polysaccharide, IOP3a from Inonotus obliquus

doi:10.7506/spkx1002-6630-201015020

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (15): 91-94.doi: 10.7506/spkx1002-6630-201015020

• Basic Research • Previous Articles Next Articles

Isolation, Purification and Anti-tumor Activity of a Polysaccharide, IOP3a from Inonotus obliquus

CHEN Yi-yong1,2，GU Xiao-hong1，TANG Jian1

1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China ；
2. School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China

Received:2009-12-28 Online:2010-08-15 Published:2010-12-29
Contact: CHEN Yi-yong1 E-mail:yiyongchen@hotmail.com

Abstract

Abstract:

Water extraction and deproteinization by Sevag method were carried out for the preparation of a crude polysaccharide extract from Inonotus obliquus, named IOP. IOP was subjected to sequential treatments with DEAE-Sepharose CL-6B anion exchange, Sepharose CL-6B and Sephadex G-200 columns for purification purpose and a polysaccharide fraction, named IOP3a, was obtained, which was identified to contain a single composition. The inhibitory effect of IOP3a on the proliferation of Jurkat and Daudi tumor cells in vitro was evaluated by MTT assay. It was found that IOP3a had an obvious anti-tumor activity in a dose-dependent manner in vitro (P ＜ 0.01) and that the maximum inhibitory ratio of IOP3a on Jurkat and Daudi tumor cells were 71.84% and 75.14%, respectively.

Key words: Inonotus obliquus polysaccharide, purification, anti-tumor activity in vitro

CLC Number:

0629.12
R73

CHEN Yi-yong1,2，GU Xiao-hong1，TANG Jian1. Isolation, Purification and Anti-tumor Activity of a Polysaccharide, IOP3a from Inonotus obliquus[J]. FOOD SCIENCE, 2010, 31(15): 91-94.

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Isolation, Purification and Anti-tumor Activity of a Polysaccharide, IOP3a from Inonotus obliquus

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