Abstract:

Fibroin protein was modified by acetylation, and some key technique parameters such as fibroin protein concentration, acetic anhydride dosage, reaction temperature, time and pH value were studied and optimized by conducting orthogonal test. Results showed that the optimum acetylation reaction was carried out at 60 ℃, pH 8.5 for 20 min with fibroin protein concentration 2% and anhydride concentration 15%. Under such conditions, the acetylation ratio was up to 47.8%.

Key words: fibroin protein, acetylation, modification