Abstract:

Optimal condition for enzymatic hydrolysis of milk protein in infant formula was investigated through evaluating nitrogen soluble index, an indictor of enzymatic hydrolysis degree, in hydrolysate solution. The optimal hydrolysis condition for protease M "Amana" G was 3 h of hydrolysis, 1.5% of enzyme-substrate ratio, 45 ℃ of reaction temperature, and pH 7.0. The nitrogen soluble index was 45.93% under this optimal condition.

Key words: milk protein, enzymatic hydrolysis, protease, infant formula