Abstract:

Objective: To develop a convenient and quick measurement method for ascorbic acid in juice. Methods: A reverse phase high performance liquid chromatography (RP-HPLC) method was established to determine the concentration of ascorbic acid in juice. The RP-HPLC was composed of SPD-10AVP as the UV-detector, Luna C18(2) column (150 mm × 4.6 mm, 5 μm) as the solid phase, 0.1% oxalic acid solution as the mobile phase with flow rate of 1.0 ml/min, UV detection wavelength at 245 nm and 30 ℃ of column oven temperature. Results: The good linear was in the range of 3.952－79.04 μg/ml (r = 0.9999) with the detection limit of 0.0007 and 0.0022 μg/ml according to 3× and 10× noise signal, respectively. The average recovery rate was 108.3% (n = 5). The RSD was 0.54 and 0.28% in precision and reproducibility tests, respectively. The determination was stable in 8 h (RSD = 5.59%). Conclusion: This RP-HPLC method is a suitable measurement method for ascorbic acid in juice due to its accuracy, convenience and quickness.

Key words: RP-HPLC, ascorbic acid, juice, determination