FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (20): 126-129.doi: 10.7506/spkx1002-6300-200920023

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Enzymolysis of Loligo japonica Protein

ZHANG Jing-min,CHEN Hong-yu   

  1. (Ocean Institute, Huaihai Institute of Technology, Lianyungang 222005, China)
  • Received:2008-10-29 Revised:2009-07-21 Online:2009-10-15 Published:2010-12-29
  • Contact: ZHANG Jing-min, E-mail:zhangjingmin929@sina.com

Abstract:

In order to optimize enzymolysis process of Loligo japonica protein, effects of protease type, enzyme dose, reaction temperature, reaction time, pH and material/water ratio on amino nitrogen (ANN) content in the hydrolysate were studied using orthogonal array design. Results showed that a maximum ANN content of 7.34 mg/g was achieved by the neutral protease hydrolysis for 4.0 h with a material/water ratio of 1:5 (m/m) and an enzyme dose of 900 U/g substrate at 50 ℃, pH 7.0.

Key words: Loligo japonica peptide, protease, amino nitrogen

CLC Number: