Construction of Industrial Brewing Yeast with High-SOD and Low-diacetyl Productivity Using Self-cloning Technique

doi:10.7506/spkx1002-6630-200919057

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (19): 248-251.doi: 10.7506/spkx1002-6630-200919057

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Construction of Industrial Brewing Yeast with High-SOD and Low-diacetyl Productivity Using Self-cloning Technique

MU Qian1，CAI Yong1，WANG Zhao-yue2，ZHANG Bo-run2，REN Zheng-long1,*

1. State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Ya'an 625014, China；2. Laboratory of
Molecular Genetics and Breeding of Yeast, Institute of Microbiology of Chinese Academy of Sciences, Beijing 100101, China

Received:2008-01-21 Online:2009-10-01 Published:2010-12-29
Contact: REN Zheng-long E-mail:qianqian84119@sina.com

Abstract

Abstract:

A recombinant plasmid pMC572 was constructed through replacing internal fragment of -acetohydroxyacid synthase (AHAS) gene (ILV2) with a fused gene that contained a copper resistant gene (CUP1), PGK1 promoter, -factor sequence and open reading frame of SOD gene from Saccharomyces cerevisiae. An industrial brewing yeast strain, YSF31, was transformed with plasmid pMC572. The self-cloning strains were selected in terms of resistance ability to CuSO4, PCR amplification assay and AHAS activity. The engineered industrial strain exhibited fermentation performance and growth properties similar to the wild type in 500 ml conical flask under identical condition of beer fermentation. Moreover, SOD produced in this industrial strain was detected in fermenting liquor and acetoin was 50% lower than that of the control. Due to DNA manipulation without heterogeneous DNA, the self-cloning strain may be acceptable.

Key words: Saccharomyces cerevisiae, SOD , acetoin , self-cloning

CLC Number:

MU Qian1，CAI Yong1，WANG Zhao-yue2，ZHANG Bo-run2，REN Zheng-long1,*. Construction of Industrial Brewing Yeast with High-SOD and Low-diacetyl Productivity Using Self-cloning Technique[J]. FOOD SCIENCE, 2009, 30(19): 248-251.

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Construction of Industrial Brewing Yeast with High-SOD and Low-diacetyl Productivity Using Self-cloning Technique

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