FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (11): 22-25.doi: 10.7506/spkx1002-6630-200911003

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Study on Extraction and Purification and Antioxidant Properties in vitro of Anthocyanins from Litchi Fruit Pericarp

HE Hui,SONG Zhe,XIE Bi-xiu,XIE Bi-jun,SUN Zhi-da*   

  1. (College of Food Science and Technology, Huazhong Agricultural University,Wuhan 430070, China)
  • Received:2008-08-23 Revised:2008-09-18 Online:2009-06-01 Published:2010-12-29
  • Contact: SUN Zhi-da* E-mail:sunzhida@mail.hzau.edu.cn

Abstract:

Anthocyanins were extracted from litchi fruit pericarp with first 60% enthanol and then ethyl acetate, and were purified with macroporous resin XAD-7HP and Sephadex LH-20 in turn. HPLC analysis showed that cyanidin-3-o-rutinoside was main component in the purified anthocyanins containing less flavonol. Total antioxidant capability and scavenging activities to hydroxyl radical, DPPH and superoxide anion of the anthocyanins were determined. Results showed that the anthocyanins had excellent antioxidant activities, and the IC50s to hydroxyl radical, DPPH radical, and superoxide anion radical were 0.69, 1.31, and 1.78 mg/L, respectively.

Key words: litchi pericarp pigment, anthocyanin, antioxidant

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