Separation and Purification of Cerebrosides from Sea Cucumbers

doi:10.7506/spkx1002-6630-200911004

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (11): 26-28.doi: 10.7506/spkx1002-6630-200911004

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Separation and Purification of Cerebrosides from Sea Cucumbers

LU Ying-tao1，KANG Cong-min1,*，XUE Chang-hu2

(1. College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China；
2. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China)

Received:2008-01-15 Revised:2009-02-04 Online:2009-06-01 Published:2010-12-29
Contact: KANG Cong-min1,*， E-mail:cmkang@qust.edu.cn

Abstract

Abstract:

Objective: The extraction and separation conditions of cerebrosides from sea cucumbers were investigated. Methods：The objective substances were extracted with mixture of chloroform and methanol, primarily isolated with petroleum ether and ethyl acetate, further isolated by silica gel column chromatography, purified by HPLC, and structurally identified by GC-MS. Results: Cerebrosides were successfully extracted and purified from sea cucumbers.

Key words: cucumber, cerebroside, extraction and isolation, HPLC

CLC Number:

TS254.1

LU Ying-tao1，KANG Cong-min1,*，XUE Chang-hu2. Separation and Purification of Cerebrosides from Sea Cucumbers[J]. FOOD SCIENCE, 2009, 30(11): 26-28.

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Separation and Purification of Cerebrosides from Sea Cucumbers

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