Determination of Aflatoxin B1 in Dry Capsicum by Enzyme-linked Immunosorbent Assay (ELISA)

doi:10.7506/spkx1002-6630-200910058

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (10): 245-247.doi: 10.7506/spkx1002-6630-200910058

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Determination of Aflatoxin B1 in Dry Capsicum by Enzyme-linked Immunosorbent Assay (ELISA)

XU Zhou1，TAN Shu-ming2,*，JIAO Yan-chao2，ZHANG Yun-fang3

(1. College of Life Science, Guizhou University, Guiyang 550025, China；2. College of Chemical Engineering, Guizhou University,
Guiyang 550004, China；3. College of Food Science, Southwest University, Chongqing 400716, China)

Received:2008-10-14 Revised:2009-01-13 Online:2009-05-15 Published:2010-12-29
Contact: XU Zhou1， E-mail:zhxu23@yahoo.com.cn

Abstract

Abstract:

In this study, aflatoxin B1 in dry capsicum was detected with ELISA kit, and the prevention and control measures of aflatoxin B1 were proposed. The results showed that the detection sensitivity was 0.1 μg/kg, the relative standard deviation (RSD) in 6 parallel determinations was less than 3% and the average spike recovery rate was 97%. This method is accurate, and has well stability and reproducibility, so it can be applied to detect effectively aflatoxin B1 in dry capsicum. Reducing oxygen content and controlling relative humidity below 80% in storage environment of dry capsicum is favorable for preventing aflatoxin B1 generation. The physical methods (adsorption, heat treatment and ray irradiation), chemical methods (oxidation and reduction, acid and alkali treatment) and biological methods (microbes and enzymes) are available to reduce aflatoxin B1 content.

Key words: ELISA, aflatoxin B1, dry capsicum

CLC Number:

TS201.6

XU Zhou1，TAN Shu-ming2,*，JIAO Yan-chao2，ZHANG Yun-fang3. Determination of Aflatoxin B1 in Dry Capsicum by Enzyme-linked Immunosorbent Assay (ELISA)[J]. FOOD SCIENCE, 2009, 30(10): 245-247.

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Determination of Aflatoxin B1 in Dry Capsicum by Enzyme-linked Immunosorbent Assay (ELISA)

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