Development of Enzyme-linked Immunoassay for Detection of Gentamicin

doi:10.7506/spkx1002-6630-200910057

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (10 ): 242-244.doi: 10.7506/spkx1002-6630-200910057

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Development of Enzyme-linked Immunoassay for Detection of Gentamicin

MA Wei，WANG Bao-huan，LI Juan，XU Chuan-lai*

(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)

Received:2008-10-30 Online:2009-05-15 Published:2010-12-29
Contact: XU Chuan-lai* E-mail:xcl@jiangnan.edu.cn

Abstract

Abstract:

An immunogen, gentamicin-bovine serum albumin (BSA), was prepared by glutaraldehyde coupling method, and was identified by SDS-PAGE. New Zealand white rabbit was immunized with this immunogen three times. After immunization the antiserum with the titer of higher than 512000 was obtained. After purified by salting-out with ammonium sulfate, the obtained anti- gentamicin antibody was used to develop an indirect enzyme-linked immunosorbent assay (ELISA) for detection of gentamicin, with the detection limit of 0.1 ng/ml.

Key words: gentamicin, antibody, immunoassy

CLC Number:

R155.5

MA Wei，WANG Bao-huan，LI Juan，XU Chuan-lai*. Development of Enzyme-linked Immunoassay for Detection of Gentamicin[J]. FOOD SCIENCE, 2009, 30(10 ): 242-244.

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Development of Enzyme-linked Immunoassay for Detection of Gentamicin

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