FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (7): 72-74.doi: 10.7506/spkx1002-6630-200907016

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Determination of Superoxide Dismutase (SOD) -like Activity of Proanthocyanidins in Seeds and Peels of  Vitaceae amurensis

ZHANG Lan-jie XIN Guang WANG Yan-li   

  1. (Department of Chemistry, Anshan Normal University, Anshan 114007, China)
  • Received:2008-06-30 Revised:2008-12-29 Online:2009-04-01 Published:2010-12-29
  • Contact: ZHANG Lan-jie E-mail:zhanglanjieas@163.com

Abstract:

Pyrogallol autooxidation method was applied to determine SOD-like activities of proanthocyanidins in seeds and peels of Vitaceae amurensis. The results indicated that the SOD-like activities of proanthocyanidins in seeds and peels of Vitaceae amurensis are 192.85 U/g and 78.45 U/g, respectively. The optimum parameters for this assay are as follows: determining absorbance at the wavelength of 325 nm, and taking Tris-HA at pH 8.2 and at the concentration of 50 mmol/L as buffer system. The inhibition percentage of proanthocyanidins against pyrogallol antooxidation reaches 91%.

Key words: Vitaceae amurensis, proanthocyanidins, superoxide dismutase activity, pyrogallol autooxidation

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