Study on Determination of Vibrio mimicus in Food by Polymerase Chain Reaction Coupled with Denaturing High Performance Liquid Chromatography (PCR-DHPLC)

doi:10.7506/spkx1002-6630-200906038

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (6): 175-177.doi: 10.7506/spkx1002-6630-200906038

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Study on Determination of Vibrio mimicus in Food by Polymerase Chain Reaction Coupled with Denaturing High Performance Liquid Chromatography (PCR-DHPLC)

ZHENG Qiu-yue1,2，CAO Ji-juan1，JIANG Dan1，LIU Shu-yan1，ZHAO Xin1，FU Jun-fan2

(1. Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China；
2. Shenyang Agricultural University, Shenyang 110161, China)

Received:2008-04-17 Revised:2008-08-08 Online:2009-03-15 Published:2010-12-29
Contact: ZHENG Qiu-yue1 E-mail:zhengqyciq@163.com

Abstract

Abstract:

PCR-DHPLC was adopted as a sensitive, specific, rapid and high flux approach for the detection of food-borne pathogenic bacteria, Vibrio mimicus in this study. DHPLC was used for separating double-stranded DNA under non-degeneration temperature and for detecting the specific PCR products. Under the optimum conditions, the clustering analysis diagram of elution peaks of samples were analyzed, and the spectrum with characteristic peak of Vibrio mimicus was obtained. The results showed that PCR-DHPLC has excellent specificity and accuracy of 100% compared with the traditional technology, therefore it has extensive application value in food safety assessment.

Key words: Vibrio mimicus, PCR, DHPLC

CLC Number:

TS201.6

ZHENG Qiu-yue1,2，CAO Ji-juan1，JIANG Dan1，LIU Shu-yan1，ZHAO Xin1，FU Jun-fan2. Study on Determination of Vibrio mimicus in Food by Polymerase Chain Reaction Coupled with Denaturing High Performance Liquid Chromatography (PCR-DHPLC)[J]. FOOD SCIENCE, 2009, 30(6): 175-177.

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Study on Determination of Vibrio mimicus in Food by Polymerase Chain Reaction Coupled with Denaturing High Performance Liquid Chromatography (PCR-DHPLC)

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