Mutation Breeding of Strain Highly Producing Androst-4-ene-3,17-dione from Rapeseed Sterols

doi:10.7506/spkx1002-6630-200905033

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (5): 145-148.doi: 10.7506/spkx1002-6630-200905033

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Mutation Breeding of Strain Highly Producing Androst-4-ene-3,17-dione from Rapeseed Sterols

YANG Ying1,2，JIANG Shao-tong1， HU Jin-yan1， ZHAO Jun-ping1，LIANG Tie-yan1

(1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China；
2. Department of Environment Engineering, Anhui Institute of Architecture, Hefei 230022, China)

Received:2008-04-15 Revised:2008-07-08 Online:2009-03-01 Published:2010-12-29
Contact: YANG Ying1,2 E-mail:yangying-sunny@sohu.com

Abstract

Abstract:

The mutagenesis of Mycobacterium sp. BD-696 was induced by UV and DES, and two mutants Mycobacterium sp. BD-696-3 and Mycobacterium sp. BD-696-6 were obtained. When the concentration of substrate steroid is 6‰ and shaking flask fermentation is kept for 168 hours, the transformation yield of androst-4-ene-3,17-dione (AD) from rapeseed sterol by Mycobacterium sp.D-696-3 and Mycobacterium sp. BD-696-6 are 2.31 g/L and 2.33 g/L, respectively, and the corresponding transformation rates are 60.7 % and 61.4%, higher 12.8% and 13.5% than that by the original strain, respectively. Moreover, the continuous passage experiment showed that the mutant BD-696-6 has high genetic stability.

Key words: rapeseed sterols, Mycobacterium sp, mutation breeding, androst-4 -ene-3,17-dione

CLC Number:

Q933

YANG Ying1,2，JIANG Shao-tong1， HU Jin-yan1， ZHAO Jun-ping1，LIANG Tie-yan1. Mutation Breeding of Strain Highly Producing Androst-4-ene-3,17-dione from Rapeseed Sterols[J]. FOOD SCIENCE, 2009, 30(5): 145-148.

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Mutation Breeding of Strain Highly Producing Androst-4-ene-3,17-dione from Rapeseed Sterols

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