Abstract:

In order to increase the production of L-lactic acid by co-fermentation of glucose and xylose, Rhizopus oryzae As3.819 was mutated by low energy N+ implantation. The results showed that the survival rate curve was a typical saddle shape with a high positive mutation rate at a dose of 50×2.5×1013 ions/cm2. Under this implantation dose, strain N50-7 was obtained with high yield and stability. L-lactic acid yield by N50-7 was 79.42 g/L, 17.75% higher than the original strain. The optimal composition of fermentation medium for N50-7 were as follows: glucose 100 g/L, xylose 50 g/L, (NH4)2SO4 3 g/L, KH2PO4 0.3 g/L, MgSO4·7H2O 0.3 g/L and ZnSO4·7H2O 0.4 g/L. The final concentration of L-lactic acid was 103.81g/L after 72 h fermentation, which was 30.71% higher than that before breeding.

Key words: ion implantation, Rhizopus oryzae, mutation breeding, mixed sugars, L-lactic acid