FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (4): 92-95.doi: 10.7506/spkx1002-6630-200904015

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Study on Ultrasonic Extraction of Hemoglobin from Pig Red Blood Cells

ZHOU Ling1,KAN Jian-quan2,WANG Xue-rong1,*   

  1. (1. Department of Animal Science, Rongchang School District, Southwest University, Chongqing 402460, China;
    2. College of Food Science, Southwest University, Chongqing 400716, China)
  • Received:2008-03-04 Revised:2008-07-20 Online:2009-02-15 Published:2010-12-29
  • Contact: WANG Xue-rong1,* E-mail:wxr13099@163.com

Abstract:

Sodium citrate was added into fresh pig blood to prepare anticoagulant blood, and then the anticoagulant blood was centrifugated to obtain red blood cells. After washing with normal saline 3 times, the red blood cells were used to extract hemoglobin by means of ultrasonic. Effects of ratio (V/V) of water to red blood cell, ultrasonic power, ratio of ultrasonic time to quiescent interval, and operation time on the extraction rate of hemoglobin were investigated. Through single factor test, their scopes were initially determined, and then the extraction technology parameters were optimized through orthogonal test. The results showed that when the ratio (V/V) of water to red blood cell is 4:1, the ultrasonic power is 100 W, the ratio of ultrasonic time to quiescent interval is 1:1, and the operation time is 20 min, the extraction rate of hemoglobin is 97.65%.

Key words: pig blood, hemoglobin, ultrasonic wave, extraction

CLC Number: