FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (3): 227-230.doi: 10.7506/spkx1002-6630-200903051

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Preliminary Study on Characteristics and Application of Chitosan-immobilized Lactase

CHEN Fang-yan1,WANG Lin-chuan2,LI Wen-chu1,WANG Ye-yuan1,ZHUO Xiao-lei1,LI Jie1   

  1. (1.College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
    2.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China)
  • Received:2008-03-04 Revised:2008-05-19 Online:2009-02-01 Published:2010-12-29
  • Contact: CHEN Fang-yan1 E-mail:chenfangyan@scau.edu.cn

Abstract:

Lactase from mung bean was immobilized onto chitosan surface by using glutaraldehyde as the cross-linking reagent. The results showed that: (1) the immobilized lactase is stable at 4 to 55 ℃; its optimum temperature and pH are 60 ℃ and 3.5, respectively; (2) its Km [ONPGal] is 0.04%, which is 0.12 times as much as that (0.33%) of free papain; (3) its operation half-life is 30 d, which is longer than that of the free lactase. Furthermore, when this chitosan-immobilized lactase is used in the plug-flow reactor to hydrolyze lactose, the hydrolysis rate is up to more than 60%.

Key words: chitosan immobilizated lactase, characteristics, lactose

CLC Number: