Gene Cloneing, Expression and Immunological Identification of Main Allergen Gal d 3 in Eggs

doi:10.7506/spkx1002-6630-200901034

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (1): 142-146.doi: 10.7506/spkx1002-6630-200901034

Previous Articles Next Articles

Gene Cloneing, Expression and Immunological Identification of Main Allergen Gal d 3 in Eggs

WU Xu-li，YANG Zhi-hua，LIU Zhi-gang*，WU Hai-qiang

(Institute of Allergy and Immunology, Shenzhen University, Shenzhen 518060, China)

Received:2007-12-06 Revised:2008-04-14 Online:2009-01-01 Published:2010-12-29
Contact: LIU Zhi-gang E-mail:lzg@szu.edu.cn

Abstract

Abstract:

Objective: To clone, express and identify the gene of Gal d 3 in eggs. Methods: The total RNA was extracted from eggs, and the gene of Gal d 3 was amplified by RT-PCR and cloned into pMD18-T for sequencing. Then the interesting gene was subcloned into pET-28a for expression. After purification of the recombinant protein, its IgE-binding capacity was determined by Western blotting. Results: The gene of Gal d 3 in eggs is obtained by clone, which has 2118 bp and encodes 705 amino acids. This isoelectric point of the encoded protein is 6.5 with a molecular weight of 76000. Conclusion: The main allergen gene Gal d 3 in eggs is successfully cloned and expressed in this study, and this recombinant protein shows good IgE-binding capacity.

Key words: eggs, allergen, clone, expression, Gal d 3

CLC Number:

WU Xu-li，YANG Zhi-hua，LIU Zhi-gang*，WU Hai-qiang. Gene Cloneing, Expression and Immunological Identification of Main Allergen Gal d 3 in Eggs[J]. FOOD SCIENCE, 2009, 30(1): 142-146 https://doi.org/10.7506/spkx1002-6630-200901034

[1]	YE Wenxin, HE Jiaxin, Vivian Montero ALEJO, YANG Meiyan. Cloning, Expression and Activity Analysis of Four Bacteriophage Lysins [J]. FOOD SCIENCE, 2026, 47(9): 128-137.
[2]	JIANG Donglei, ZHANG Sidi, XU Yang, WANG Ke, WANG Lifeng. A 5-Hydroxytryptamine Release-Based Biomimetic Microtissue Sensor for Detecting Creatine Kinase [J]. FOOD SCIENCE, 2026, 47(9): 333-340.
[3]	ZENG Yajuan, ZHANG Zixuan, LI Xuan, CAI Lehong, ZHOU Haiyan. Highly Efficient Heterologous Expression of the Sweet Protein Mabinlin II (M12) from Capparis masaikai Levl in Hairy Root System of Brassica napus L. [J]. FOOD SCIENCE, 2026, 47(7): 159-165.
[4]	GAO Xue, XING Weihai, YU Yanan, ZHANG Junmin, YANG Youyou, CAO Riliang. Differentiating Odor Compounds of Common Poultry Eggs Based on Gas Chromatography-Olfactometry-Orbitrap High-Resolution Mass Spectrometry [J]. FOOD SCIENCE, 2026, 47(7): 211-220.
[5]	WANG Jin, TANG Yajie, ZHANG Lili, WANG Rui, SUN Quancai. Research Progress in the Analysis and Detection of Peanut Allergens in Foods: From Traditional Methods to Novel Biosensors [J]. FOOD SCIENCE, 2026, 47(4): 1-16.
[6]	ZHANG Haichao, WEI Xinxin, ZHANG Jingwen, JIA Haitao, AI Lianfeng, WANG Jing, HUANG Xuejing, QIN Xiaohang, LIU Baoru. Fast Determination of Diclazuril and Toltrazuril Residue Makers in Eggs by Ultra-high Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Triple Quadrupole Mass Spectrometry [J]. FOOD SCIENCE, 2025, 46(8): 330-339.
[7]	WENG Xiaomin, HU Shiqi, CAI Jiaqi, HONG Jianqu, YAN Fen. Heterologous Expression, Enzymatic Characterization, and Biofilm Eradication Activity of Cellulase CelL7 Derived from Marine Sources [J]. FOOD SCIENCE, 2025, 46(6): 124-132.
[8]	XIANG Cong, ZHONG Mengzhen, HUO Jiaying, LI Shugang, MA Lulu. Effects of Storage Conditions on Gel Characteristics of Soft-Boiled Egg White and Underlying Mechanisms [J]. FOOD SCIENCE, 2025, 46(5): 281-291.
[9]	XU Jingwen, WU Rina, WANG Weiming, YAN Yiran, XIANG Xueqin, ZHAO Yulian, WU Junrui, WANG Yaqi, DENG Li, TONG Xing, SHI Haisu. Heterologous Expression of the Fibrinolytic Enzyme Gene from Bacillus velezensis 20-1, Isolated from Sojae Semen Praeparatum and Application of Recombinant Strain Containing It in the Production of Sojae Semen Praeparatum [J]. FOOD SCIENCE, 2025, 46(4): 68-80.
[10]	WEI Zhen, CHEN Jinling, XU Linxiang. Bioinformatics Analysis, Heterologous Expression and Enzymatic Characterization of Dextranase from Streptococcus thermophiles LMG 18311 [J]. FOOD SCIENCE, 2025, 46(22): 246-256.
[11]	NIU Zehui, LIANG Zhihong. Current Advances in Ochratoxin A-Degrading Enzymes [J]. FOOD SCIENCE, 2025, 46(20): 370-382.
[12]	XUE Yibin, Zeng Longda, YAN Qiaojuan, JIANG Zhengqiang. High-Level Expression, Characterization and Application of a Novel Aspartic Protease from Aspergillus niger [J]. FOOD SCIENCE, 2025, 46(20): 100-110.
[13]	XUE Zhenglian, WANG Yuqing, LI Chuang, LI Dandan, ZHU Sibao, LI Xiangfei. Heterologous Expression and Transglycosylation of Acarviosyltransferase [J]. FOOD SCIENCE, 2025, 46(2): 81-88.
[14]	PENG Jingjing, YUE Shiyang, LU Lianghua, LIU Xiaoling, WANG Chenghua. Mechanism of Selenoprotein Expression and Enzymatic Activity Characteristics in Natural Lactococcus lactis [J]. FOOD SCIENCE, 2025, 46(19): 116-123.
[15]	YANG Yuying, LI Yanchu, LIU Jiatong, WANG Zefu, HAN Zongyuan, LIU Shucheng, WEI Shuai. Effect of Combined Ultrasound and High Pressure CO2 Treatment on the Structure and Allergenicity of Shrimp Tropomyosin [J]. FOOD SCIENCE, 2025, 46(16): 295-303.

Gene Cloneing, Expression and Immunological Identification of Main Allergen Gal d 3 in Eggs

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments