FOOD SCIENCE ›› 2025, Vol. 46 ›› Issue (16): 295-277.doi: 10.7506/spkx1002-6630-20250110-083

• Food Engineering • Previous Articles     Next Articles

Effect of Combined Ultrasound and High Pressure CO2 Treatment on the Structure and Allergenicity of Shrimp Tropomyosin

YANG Yuying, LI Yanchu, LIU Jiatong, WANG Zefu, HAN Zongyuan, LIU Shucheng, WEI Shuai   

  1. (1. Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China; 2. Liaoning Marine Food Deep Processing Key Technology Provincial Co-construction Collaborative Innovation Center, Dalian Polytechnic University, Dalian 116034, China)
  • Published:2025-07-22

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Abstract: The combined effect of ultrasound and high pressure carbon dioxide (HPCD) on the structure and allergenicity of the tropomyosin (TM) of Litopenaeus vannamei was studied. After sequential treatment with ultrasonic at powers up to 2 000 W for up to 60 min followed by HPCD at 30 MPa for 15 min, the allergenicity of TM was detected by indirect enzyme-linked immunosorbent assay (iELISA) and Western blot (WB), and the molecular mass was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The spatial structure of TM was characterized by circular dichroism (CD) spectroscopy, fluorescence spectroscopy, ultraviolet (UV) spectroscopy, free amino group and total sulfhydryl group analysis. The results showed that ultrasound at 1 500 W for 30 min combined with HPCD decreased the immunobinding activity of immunoglobulin G (IgG) and IgE in TM by 55.3% and 48.7%, respectively, the α-helix relative content decreased to 42.7%, and the secondary structure was changed from an ordered state to a disordered state. The fluorescence spectra showed a blue shift with weakened fluorescence intensity, and the UV spectra exhibited a red shift with enhanced absorption intensity. The free amino group content was reduced by 21.82%, and the total sulfhydryl group content was also reduced. Therefore, ultrasound combined with HPCD treatment changed the spatial conformation of TM, being more effective in reducing the allergenicity than either single treatment.

Key words: Litopenaeus vannamei; tropomyosin; ultrasound combined with high pressure CO2; structure; allergenicity

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