FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (1): 211-213.doi: 10.7506/spkx1002-6630-200901050

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Molecular Cloning and Sequence Analysis of Partial 18SrDNA of Mold in Koji

LI Zai-xin1,2,LEI Yun-ling1,LIU Da-yu1,XIE Wan-ru1,XIANG Chao1   

  1. (1.Department of Biotechnology Engineering, Sichuan University of Science and Engineering, Zigong 643000, China;
    2. College of Bioengineering, Chongqing University, Chongqing 400044, China)
  • Received:2008-01-08 Revised:2008-09-12 Online:2009-01-01 Published:2010-12-29
  • Contact: LI Zai-xin1 E-mail:lizaixin@sina.com

Abstract:

Genome DNA extracted from mold in koji was acted as model in the partial 18SrDNA sequence-amplified reaction by PCR. The DNA sequences were inserted into screening vector pKRX-T, and then were transformed into E.coil HB101. The results of sepuencing and simlarity analysis showed that the mold strain is similar to Rhizopus oryzae at 99 %. This strain might be regarded as mutant of Rhizopus oryzae.

Key words: 18SrDNA, Rhizopus oryza, koji

CLC Number: