Abstract:

Endophytes are a very important resource of microorganisms with potential application values. A novel fibrinolytic enzyme (PPFE-I) was purified from the fermentation supernatant of endophytic bacterium Paenibacillus polymyxa EJS-3 by the following procedure including ammonium sulfate precipitation, Hiprep phenyl FF hydrophobic chromatography, RESOURCEMQ ion exchange chromatography and Sephacryl S-300HR gel filtration. The apparent molecular mass of PPFE-I was estimated to be 63 kDa by SDS-PAGE analysis. The purity of PPFE-I was 94.1% determined by HPLC. Using the above procedure, 16 mg of active proteins were obtained from 1 L of the fermentation supernatant of Paenibacillus polymyxa EJS-3 and a 14.5-fold increase in the specific activity of PPFE-I was achieved and the activity recovery was 3.3%. Meanwhile, the final purified product had good thrombolytic activity in vitro.

Key words: endophytes, Paenibacillus polymyxa, fibrinolytic enzyme, purification, thrombolytic effect