Isolation, Purification and Relative Molecular Mass Determination of Corn Bran Polysaccharides

doi:10.7506/spkx1002-6630-201010004

Abstract

Abstract:

Crude corn bran polysaccharides (CBP) were obtained through microwave-assisted extraction followed by 95% alcohol precipitation, purified through D315 ion exchange resin column chromatography for the removal of protein and fractionated into 2 fractions, CBPA and CBPB, through fractional precipitation with alcohol. CBPA was further fractionated through Sephadex G-100 column chromatography into 2 subfractions, CBPA-1 and CBPA-2 of which Sephadex G-75 column elution profile exhibited a single absorption peak. Sephadex G-75 column chromatographic analysis indicated that CBPB comprised a single homogeneous composition. Neither protein nor nuclear acid characteristic absorption peaks were shown in the UV-visible spectra of CBPA-1, CBPA-2 and CBPB. The relative molecular mass of CBPA-1, CBPA-2 and CBPB were determined by the dextran gel filtration method to be 186170, 103885 and 146887 , respectively.

Key words: corn spermoderm, polysaccharide, purification, relative molecular mass

CLC Number:

S513

ZHANG Yan-rong，YU Jun，LIU Xiang-yang，WANG Da-wei*. Isolation, Purification and Relative Molecular Mass Determination of Corn Bran Polysaccharides[J]. FOOD SCIENCE, 2010, 31(10 ): 16-19.

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Isolation, Purification and Relative Molecular Mass Determination of Corn Bran Polysaccharides

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