FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (10 ): 16-19.doi: 10.7506/spkx1002-6630-201010004

• Processing Technology • Previous Articles     Next Articles

Isolation, Purification and Relative Molecular Mass Determination of Corn Bran Polysaccharides

ZHANG Yan-rong,YU Jun,LIU Xiang-yang,*   

  1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China
  • Received:2010-03-02 Online:2010-05-15 Published:2010-12-29
  • Contact: WANG Da-wei


Crude corn bran polysaccharides (CBP) were obtained through microwave-assisted extraction followed by 95% alcohol precipitation, purified through D315 ion exchange resin column chromatography for the removal of protein and fractionated into 2 fractions, CBPA and CBPB, through fractional precipitation with alcohol. CBPA was further fractionated through Sephadex G-100 column chromatography into 2 subfractions, CBPA-1 and CBPA-2 of which Sephadex G-75 column elution profile exhibited a single absorption peak. Sephadex G-75 column chromatographic analysis indicated that CBPB comprised a single homogeneous composition. Neither protein nor nuclear acid characteristic absorption peaks were shown in the UV-visible spectra of CBPA-1, CBPA-2 and CBPB. The relative molecular mass of CBPA-1, CBPA-2 and CBPB were determined by the dextran gel filtration method to be 186170, 103885 and 146887 , respectively.

Key words: corn spermoderm, polysaccharide, purification, relative molecular mass

CLC Number: