Abstract:

The active ingredients from above-ground parts were prepared using aqueous extraction and solvent extraction including ethanol and petroleum ether. The performing condition for solvent extraction of flavonoids was optimized by an orthogonal experiment design and the factors influencing the yields of flavonoids were also investigated including extraction time, temperature, solvent concentration and liquid/solid ratio. The results suggested the abundance of active ingredients in aboveground part of Clematis sibirica (L.) Mill. Analyses on the contents of flavonoids in the extracts from various parts of Clematis sibirica (L.) Mill showed that all the parts were rich in flavonoids while the contents varied and the order decreased as follows: leaf >fruit> stalk.

Key words: Clematis sibirica (L.) Mill., active ingredients, total flavonoids, determination