Abstract:

The high-copy-number plasmid PBJPIM, containing the S.aureofuscus PimM gene under the promoter PermE , was constructed. This fragment was ligated with pSET152, resulting in plasmid PBJPIM, and the plasmid PBIPIM was introduced into wild-type S.aureofuscus SYAU0709 via E.coli — Streptomyces aureofuscus conjugation. The aureofuscin yield was found to be increased by 3 — 4 times in some transformants when compared with that in the strain SYAU0709. Our study demonstrates that PimM gene can promote aureofuscin biosynthesis and the recombinant strains obtained have highly stable heritage.

Key words: Streptomyces aureofuscus, aureofuscin, PimM gene