Abstract: C-phycocyanin enrichment by salting-out and iso-electric precipitation was carried out. Effects of various parameters such as Spirulina powder-phosphate buffer ratio (m/V), salt concentration, pH and temperature on C-phycocyanin yield and separation coefficient were studied. The stability of C-phycocyanin was studied too. Results showed that the separation coefficient of C-phycocynain is as high as 1.38 with a yield of 60.0 mg/g isolated by salting-out precipitation with biomass-solvent ratio of 0.5 g/100 ml and 50% (W/V) (NH4)2SO4. C-phycocyanin is steady under 30 ℃, but it denatures in above 40 ℃ environment. C-phycocyanin is steady on condition of neutral pH value.

Key words: Spirulina, C-phycocyanin, salting-out, stability, yield