Abstract: The regularity of sterilization to Staphylococcus Aureus, Escherichia Coli O157:H7, Listeria Monocytogenes and saprophytic yeast on apple surface with chlorine dioxide gas were studied. Result showed that under the conditions of 25 ℃, sterilization time 5 min and the 99.99% bactericidal rate to Staphylococcus aureus, Escherichia coli, Listeria monocytogenes and saprophytic yeast can be achieved at the concentration of chlorine dioxide gas 23, 21, 6 and 11 mg/L, respectively. Under the conditions of 25 ℃ and concentration of chlorine dioxide gas 10 mg/L, all bactericidal rates to the mentioned above four bacteria are over 99.99% at 7, 8, 2 and 6.5 min, respectively. In the experimental range, the effect of bactericidal temperature is weak.

Key words: Staphylococcus aureus, Escherichia coli O157:H7, Listeria monocytogenes, saprophytic yeast, chlorine dioxide gas, apple