Preparation and Purification and Amino Acid Sequence Analysis of Bovine Lactoferricin

Abstract

Abstract: The method of preparation and Isolation and purification of bovine lactoferricin(LfcinB) was studied. Hydrolysate of lactoferrin by pepsin was separated and purified by different medium, and the purity of lactofrricin was detected by capillary electrophoresis method. The amino acid compose of lactoferricin was analyzed by automatic amino acid analyzer, and the amino acid sequence was confirmed by the method of goodness of fit test. The results showed that preparation purity of lactoferrcin by Butyl-Toyopearl 650M is 95.6%. The amino acid compose and sequnce of the purification production there is no significant difference comparison for known LfcinB.

Key words: lactoferrin, lactoferricin, isolation and purification, acid sequence analysis

HU Zhi-He, FENG Fei, LIU Chuan-Guo, PANG Guang-Chang. Preparation and Purification and Amino Acid Sequence Analysis of Bovine Lactoferricin[J]. FOOD SCIENCE, 2008, 29(7): 166-170.

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Preparation and Purification and Amino Acid Sequence Analysis of Bovine Lactoferricin

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