Abstract: Objective: To detect TLR4 expression on protein and gene levels synchronously, investigate the effects of DVL (Daohuaxiang vigor liquor) and DCL (Daohuaxiang common liquor) on TLR4 expression in hepatic tissue of rat for differentiat-ing of differences of their toxicity, and probe into the mechanism of toxicity and liver injury induced by alcohol. Methods: 28 SD rats were randomly divided into DVL group, DCL group and normal control group. The rat model of acute liver injury was established by administration with high dose of above liquors. The expression of TLR4 on protein and gene levels in hepatic tissue was measured by immunohistochemistry and real-time RT-PCR methods. Results: The results of immunohistochemistry and RT-PCR both demonstrated that TLR4 expression of hepatic tissue is obviously increased in DCL group. However, the expression amount of TLR4 in DVL group is significantly lower than that in DCL group. Conclusion:DVL can obviously decline the TLR4 expression level in hepatic tissue so as to relieve the liver injury compared with DCL.

Key words: Daohuaxiang vigor liquor (DVL), TLR4 (Toll-like receptor 4), liver injury