Extraction and Purification of Bacteriocin from Pediococcus acidilactici Strain

Abstract

Abstract: Fresh culture of Pediococcus acidilactici strain inoculated in MRS broth and cultivated under 37 ℃ for 17 h, the bacteriocin was extracted from the culture and purified by three steps:the pH mediated cell adsorption-desorption method, SP Sepharose Fast Flow ion-exchange chromatography and Sephadex G-50 gel filtration. The final yield of bacteriocin was 0. 19% and the specific activity was 5.17×104 AU/mg. The specific activity was increased by 34.54 fold. Tricine-SDS-PAGE showed that its molecular weight was about 4600 D.

Key words: bacteriocin, extraction, purification, Tricine-SDS-PAGE

LI Ya-Ling, ZHOU Zhi-Jiang, HAN Ye, WANG Pei, DING Cheng-Wei, BAI He. Extraction and Purification of Bacteriocin from Pediococcus acidilactici Strain[J]. FOOD SCIENCE, 2008, 29(2): 271-274.

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Extraction and Purification of Bacteriocin from Pediococcus acidilactici Strain

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