Abstract: To better understand the molecular mechanism of honeybee pollen at the protein level during storage, two-dimensional gel electrophoresis technology was used to investigate honeybee pollen of Camellia sinensis stored at room temperature and at -20 ℃ for 6 months respectively. Result showed that 261 protein spots are separated from honeybee pollen stored at room temperature, while 396 protein spots are detected from honeybee pollen stored at -20 ℃. The reducing number of total proteins in honeybee pollen stored at room temperature is less than that at -20 ℃. Moreover, results indicated that storage of honeybee pollen at room temperature might causes protein degradation. Simultaneously, the abundance of certain protein is different at different storage temperatures. 24 proteins abundances are higher in honeybee pollen stored at room temperature than that at -20 ℃. Furthermore, 112 protein spots show lower abundance in honeybee pollen stored at room temperature than that at -20 ℃. Results indicated that protein and protein abundance might be affected by storage temperature. honeybee pollen storing at -20 ℃ is probably a better storage method to maintain its quality.

Key words: honeybee pollen of Camellia sinensis, two-dimensional gel electrophoresis, proteome, storage temperature