Determination of Vitamin E and Sterols in Refined Products from Deodorizer Distillate by High Performance Liquid Chromatography

Abstract

Abstract: A high performance liquid chromatographic method for the determination of vitamin E and sterols has been established. Separation was carried out on a C18 column (Atlantics column) at 30℃ with mobile phase of methanol and water(96:4, V/V), and PDA detection for vitamin E at 292nm and for sterols at 205nm. The experimental results showed that there is a good linear relationship between the peak area and the concentration of components. The average recoveries and relative standard deviation were well showed. The results showed that the method is simple, rapid and accurate for determination of vitamin E and sterols ind eodorizerd istillate.

Key words: deodorizer distillate, VE, sterol, high performance liquid chromatography

SHAO Ping, JIANG Shao-Tong, PAN Li-Jun, CHEN Xiao-Yan. Determination of Vitamin E and Sterols in Refined Products from Deodorizer Distillate by High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2007, 28(1): 229-231.

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Determination of Vitamin E and Sterols in Refined Products from Deodorizer Distillate by High Performance Liquid Chromatography

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