FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (7): 60-62.

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Study on Spectral Characteristics of Alliinase

 LI  Yu, XU  Shi-Ying, PANG  Ling-Yun, QIAO  Ming-Wu   

  1. 1.College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China; 2.School of Food Science and Technology, Southern Yangtze University, Key Laboratory of Food Science and Safety, Ministry of Education, Wuxi 214036, China
  • Online:2007-07-15 Published:2011-10-24

Abstract: In this paper, alliinase from fresh garlic was purified with Sephacryl S-200 gel column and the spectral characteristic of ultraviolet/visible spectra, fluorescence and CD spectroscopy were studied. The results showed that in alliinase α-helix structure accounts for about 27%~30%, β-pleated sheet structure almost doesn’t exist, β-turn structure accounts for about 30%, and random coil structure accounts for about 40%.

Key words: garlic, alliinase, spectral characteristics