Abstract: With corn embryo being coproduct in corn starch production as material, glutathione (GSH) was extracted through hot water extraction after supercritical CO2 fluid extraction and microwave treatment. The results showed that optimum conditions of supercritical CO2 fluid extraction are pressure 25 MPa, temperature 40 ℃, time 120 min, CO2 flow 20 L/g; On these conditions the extraction rate of fat is 57.03%, and fat concent of embryo dregs is 0.72%. The optimum conditions of hot water extraction are solid:liquid 1:20, temperature 90 ℃, pH3.0, time 20 min. On these conditions GSH yield is 104 mg/100 g corn embryo.

Key words: corn embryo, glutathione, supercritical fluid extraction, hot water extraction