Abstract:  Objective: By bio-fermentation engineering to expand production of Ganoderma lucidum polysaccharide. Method: Minor farm products (such as maize powders, soybean powders) were used as culture media, after controlling parameters in fermenter (such as inoculation quantity, total saccharide quantity, pressure of fermenter, temperature and growth factor etc.) to increase production. Result: Twenty normal tests were studied. The volume of every fermenter was 10m3, the fermentation liquid 7.5m3, the average production cycle 150h and pH reduced from 6.5 to 3.5. After the mycelia fermented, the average weight of dry powders in every fermenter is 66.1kg by spray drying, the average recovery 8.76kg/m3, the content of pure polysaccharide 68.5g/kg in the dry powders and the weight of the pure polysaccharide 4.225kg in every fermenter. There was no obvious vgerminate and lock like connection on the mycelia wall. There was a little mycelia dissolved with no other bacteria. Conclusion: It proves that the technology is successful. It is valuable to popularize.

Key words: Ganoderma lucidum polysaccharide, Ganoderma lucidum mycelia, bio-fermentation